negative selection stemcell cat no. Search Results


adenosine  (Chem Impex International)
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Chem Impex International adenosine
Adenosine, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stemsep negative selection human b-cell enrichment kit antibody cocktail (cat. #14064a)  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc stemsep negative selection human b-cell enrichment kit antibody cocktail (cat. #14064a)
Stemsep Negative Selection Human B Cell Enrichment Kit Antibody Cocktail (Cat. #14064a), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human t cell enrichment kit stemcell #cat: 19051  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc human t cell enrichment kit stemcell #cat: 19051
Human T Cell Enrichment Kit Stemcell #Cat: 19051, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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negative selection neutrophil isolation kit cat no.19666  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc negative selection neutrophil isolation kit cat no.19666
a A representative image of naïve dHL-60 cells incubated in nanowells formed by laser micropatterning of poly(ethylene glycol) diacrylate hydrogel within one microwell of a 384-well plate. Scale bar: 100 µm. b dHL-60 cells stained with Hoechst were treated with either ionomycin (5 µM) or culture medium (control) at baseline (0 h) and after 4.5 h. Scale bar: 100 µm. c Representative microscopy images showing NET induction in dHL-60 cells treated with ionomycin (1 µM) for 2 h. Scale bar: 20 µm. d Percentage of dHL-60 cells producing NETs after ionomycin stimulation (6 µM, 4.5 h) relative to the averaged density of total cells incubated in nanowells. e Percentage of human <t>neutrophils</t> producing NETs after ionomycin (5 µM, 4.5 h) or LPS stimulation (20 µg/mL, 4.5 h) relative to the averaged density of total cells incubated in nanowells. All P values < 0.001.
Negative Selection Neutrophil Isolation Kit Cat No.19666, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/negative selection neutrophil isolation kit cat no.19666/product/STEMCELL Technologies Inc
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immunomagnetic negative selection kit cat# 19055  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc immunomagnetic negative selection kit cat# 19055
a A representative image of naïve dHL-60 cells incubated in nanowells formed by laser micropatterning of poly(ethylene glycol) diacrylate hydrogel within one microwell of a 384-well plate. Scale bar: 100 µm. b dHL-60 cells stained with Hoechst were treated with either ionomycin (5 µM) or culture medium (control) at baseline (0 h) and after 4.5 h. Scale bar: 100 µm. c Representative microscopy images showing NET induction in dHL-60 cells treated with ionomycin (1 µM) for 2 h. Scale bar: 20 µm. d Percentage of dHL-60 cells producing NETs after ionomycin stimulation (6 µM, 4.5 h) relative to the averaged density of total cells incubated in nanowells. e Percentage of human <t>neutrophils</t> producing NETs after ionomycin (5 µM, 4.5 h) or LPS stimulation (20 µg/mL, 4.5 h) relative to the averaged density of total cells incubated in nanowells. All P values < 0.001.
Immunomagnetic Negative Selection Kit Cat# 19055, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunomagnetic negative selection kit cat# 19055/product/STEMCELL Technologies Inc
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negative selection stemcell cat no. #15063  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc negative selection stemcell cat no. #15063
a A representative image of naïve dHL-60 cells incubated in nanowells formed by laser micropatterning of poly(ethylene glycol) diacrylate hydrogel within one microwell of a 384-well plate. Scale bar: 100 µm. b dHL-60 cells stained with Hoechst were treated with either ionomycin (5 µM) or culture medium (control) at baseline (0 h) and after 4.5 h. Scale bar: 100 µm. c Representative microscopy images showing NET induction in dHL-60 cells treated with ionomycin (1 µM) for 2 h. Scale bar: 20 µm. d Percentage of dHL-60 cells producing NETs after ionomycin stimulation (6 µM, 4.5 h) relative to the averaged density of total cells incubated in nanowells. e Percentage of human <t>neutrophils</t> producing NETs after ionomycin (5 µM, 4.5 h) or LPS stimulation (20 µg/mL, 4.5 h) relative to the averaged density of total cells incubated in nanowells. All P values < 0.001.
Negative Selection Stemcell Cat No. #15063, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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positive-selection kits cat. no:17851&17854  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc positive-selection kits cat. no:17851&17854
a A representative image of naïve dHL-60 cells incubated in nanowells formed by laser micropatterning of poly(ethylene glycol) diacrylate hydrogel within one microwell of a 384-well plate. Scale bar: 100 µm. b dHL-60 cells stained with Hoechst were treated with either ionomycin (5 µM) or culture medium (control) at baseline (0 h) and after 4.5 h. Scale bar: 100 µm. c Representative microscopy images showing NET induction in dHL-60 cells treated with ionomycin (1 µM) for 2 h. Scale bar: 20 µm. d Percentage of dHL-60 cells producing NETs after ionomycin stimulation (6 µM, 4.5 h) relative to the averaged density of total cells incubated in nanowells. e Percentage of human <t>neutrophils</t> producing NETs after ionomycin (5 µM, 4.5 h) or LPS stimulation (20 µg/mL, 4.5 h) relative to the averaged density of total cells incubated in nanowells. All P values < 0.001.
Positive Selection Kits Cat. No:17851&17854, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/positive-selection kits cat. no:17851&17854/product/STEMCELL Technologies Inc
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immunomagnetic negative selection stemcell cat#19861  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc immunomagnetic negative selection stemcell cat#19861
a A representative image of naïve dHL-60 cells incubated in nanowells formed by laser micropatterning of poly(ethylene glycol) diacrylate hydrogel within one microwell of a 384-well plate. Scale bar: 100 µm. b dHL-60 cells stained with Hoechst were treated with either ionomycin (5 µM) or culture medium (control) at baseline (0 h) and after 4.5 h. Scale bar: 100 µm. c Representative microscopy images showing NET induction in dHL-60 cells treated with ionomycin (1 µM) for 2 h. Scale bar: 20 µm. d Percentage of dHL-60 cells producing NETs after ionomycin stimulation (6 µM, 4.5 h) relative to the averaged density of total cells incubated in nanowells. e Percentage of human <t>neutrophils</t> producing NETs after ionomycin (5 µM, 4.5 h) or LPS stimulation (20 µg/mL, 4.5 h) relative to the averaged density of total cells incubated in nanowells. All P values < 0.001.
Immunomagnetic Negative Selection Stemcell Cat#19861, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunomagnetic negative selection stemcell cat#19861/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
immunomagnetic negative selection stemcell cat#19861 - by Bioz Stars, 2026-02
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Image Search Results


a A representative image of naïve dHL-60 cells incubated in nanowells formed by laser micropatterning of poly(ethylene glycol) diacrylate hydrogel within one microwell of a 384-well plate. Scale bar: 100 µm. b dHL-60 cells stained with Hoechst were treated with either ionomycin (5 µM) or culture medium (control) at baseline (0 h) and after 4.5 h. Scale bar: 100 µm. c Representative microscopy images showing NET induction in dHL-60 cells treated with ionomycin (1 µM) for 2 h. Scale bar: 20 µm. d Percentage of dHL-60 cells producing NETs after ionomycin stimulation (6 µM, 4.5 h) relative to the averaged density of total cells incubated in nanowells. e Percentage of human neutrophils producing NETs after ionomycin (5 µM, 4.5 h) or LPS stimulation (20 µg/mL, 4.5 h) relative to the averaged density of total cells incubated in nanowells. All P values < 0.001.

Journal: Communications Biology

Article Title: Auto-amplification and spatial propagation of neutrophil extracellular traps

doi: 10.1038/s42003-024-06074-z

Figure Lengend Snippet: a A representative image of naïve dHL-60 cells incubated in nanowells formed by laser micropatterning of poly(ethylene glycol) diacrylate hydrogel within one microwell of a 384-well plate. Scale bar: 100 µm. b dHL-60 cells stained with Hoechst were treated with either ionomycin (5 µM) or culture medium (control) at baseline (0 h) and after 4.5 h. Scale bar: 100 µm. c Representative microscopy images showing NET induction in dHL-60 cells treated with ionomycin (1 µM) for 2 h. Scale bar: 20 µm. d Percentage of dHL-60 cells producing NETs after ionomycin stimulation (6 µM, 4.5 h) relative to the averaged density of total cells incubated in nanowells. e Percentage of human neutrophils producing NETs after ionomycin (5 µM, 4.5 h) or LPS stimulation (20 µg/mL, 4.5 h) relative to the averaged density of total cells incubated in nanowells. All P values < 0.001.

Article Snippet: To obtain purified human neutrophils and dHL-60 cells, a negative selection neutrophil isolation kit (Cat No.19666, 18000, Stemcell Technologies) was used by following the manufacturer’s instructions.

Techniques: Incubation, Staining, Control, Microscopy

a Schematic of the secondary NETosis assay in nanowells where naïve cells were incubated with cells pre-stimulated with ionomycin (6 µM) in clean media. The secondary NETosis was quantified by the measurement of NETs release in naïve cells: The NETs release was evaluated by Hoechst staining of naïve cells and measured as the percentage of cells undergoing NETosis. NETs release by ( b ) dHL-60 cells and ( c ) primary neutrophils after co-incubating with ionomycin-stimulated cells at varying cell densities. d Secondary NETosis in primary neutrophils after different treatments on stimulated cells: stimulated cells (no treatment), cell-free NETs isolation, ODA-A151 treatment, DNase I treatment, Proteinase K treatment. All statistical analyses were carried out using one-way ANOVA with Tukey’s post-hoc test * p < 0.05; ** p < 0.01; *** p < 0.001. All the values shown were averaged from three individual experiments. Error bars represent standard deviation.

Journal: Communications Biology

Article Title: Auto-amplification and spatial propagation of neutrophil extracellular traps

doi: 10.1038/s42003-024-06074-z

Figure Lengend Snippet: a Schematic of the secondary NETosis assay in nanowells where naïve cells were incubated with cells pre-stimulated with ionomycin (6 µM) in clean media. The secondary NETosis was quantified by the measurement of NETs release in naïve cells: The NETs release was evaluated by Hoechst staining of naïve cells and measured as the percentage of cells undergoing NETosis. NETs release by ( b ) dHL-60 cells and ( c ) primary neutrophils after co-incubating with ionomycin-stimulated cells at varying cell densities. d Secondary NETosis in primary neutrophils after different treatments on stimulated cells: stimulated cells (no treatment), cell-free NETs isolation, ODA-A151 treatment, DNase I treatment, Proteinase K treatment. All statistical analyses were carried out using one-way ANOVA with Tukey’s post-hoc test * p < 0.05; ** p < 0.01; *** p < 0.001. All the values shown were averaged from three individual experiments. Error bars represent standard deviation.

Article Snippet: To obtain purified human neutrophils and dHL-60 cells, a negative selection neutrophil isolation kit (Cat No.19666, 18000, Stemcell Technologies) was used by following the manufacturer’s instructions.

Techniques: Incubation, Staining, Isolation, Standard Deviation